Effects of Atrazine (Herbicide) on Blood Biochemical Indices of Grass Carp (Ctenopharhyngoden idella)

Atrazine is currently the most widely used herbicide in agriculture for the production of corn and other crops in the world. In the present research, the effect of acute and chronic toxicity of atrazine (herbicide) on blood biochemical indices of grass carp (Ctenopharhyngoden idella) an important species of commercial fish was studied. In acute toxicity, pollutant toxicity to fish was tested by using lethal concentrations and determining the LC50 (lethal concentrations for 50% of fishes). Obtained results from PROBIT analysis showed 96h-LC50 values at 80 mg/L of atrazine. In chronic toxicity, the fish were exposed at different concentrations of atrazine (0, 10, 100, 1000, 10,000 μg/L with three triplicates for each treatment) for a month (30 days). Sampling was carried out in three times (10th, 20th and 30th days). The results showed that plasma total protein, albumin, glucose, cholesterol and triglycerides decreased significantly (P<0.05) with increasing concentrations and days. Results also showed that minimum mortality rate of fish belonged to control (0%) and 10 μg/L and maximum mortality was belonging to 10000 μg/L up to the 10th day and 1000 μg/L up to 20th day and reached 100%. Therefore, these parameters could be used as an index for evaluation of pathological conditions of this fish species.